Features of obtaining an aseptic culture of Prunus dulcis (Mill.) D.A. Webb.

The purpose of the presented article is to establish features obtaining an aseptic culture regenerants Prunus dulcis in vitro. Since impact climate change becoming increasingly large-scale Ukraine, one urgent problems that hinders humanity, both terms agro-ecology and food, need diversify traditional agriculture. Mydal valuable promising nut crops thanks which it possible climatic risks listed above. varieties domestic selection were taken for experiments, since foreign with low winter resistance a long growing season are not suitable our zone. Four intensive new almonds E5 Borozan, M41 Alex, Georgia, Louise, bred by breeder V.M. Babanskyi entered into State Register Plant Varieties, allowed Ukraine cultivation. One reliable methods reproduction microclonal reproduction, help quickly obtain high-quality, disease-free planting material. Therefore, production material, modern nurseries switching biotechnological methods. It was established preparation explant donors reduces number primary explants released phenolic substances. In addition donors, nutrients, present different amounts nutrient media composition, had significant impact. fewest phenol-like exudate on NAM NRM media.Common these two environments relatively content nitrogen ammonium nitrate forms, DKW environment contains highest amount sulfur. release also depended biological characteristics almond varieties. high-growing variety least Louise medium growth intensity. Depending variety, 81–91 % free from contaminants depository, while 59–70 control. Morphogenic at same time without signs contamination found 69 among up 73 Borozan compared 35 51 %, respectively, Based results research, influence isolation interaction plant its surrounding microbiota seasons different. This manifested objects and, accordingly, success decontamination (E1) appearance microbiological medium. Key words: propagation, crops, multiplication, morphogenesis, contaminants, decontamination.